anti human βactin antibody  (Cell Signaling Technology Inc)

Journal: Scientific reports

Article Title: High androgen level during controlled ovarian stimulation cycle impairs endometrial receptivity in PCOS patients.

doi: 10.1038/s41598-024-74295-7

Figure Lengend Snippet: Fig. 1. Expression of endometrial receptivity marks in infertile women with tubal factor and women with polycystic ovary syndrome (PCOS). (a) Messenger RNA expressions of insulin-like growth factor binding protein 1 (IGFBP-1), epidermal growth factor (EGF), transforming growth factor-beta (TGF β1), leukemia inhibitory factor (LIF), matrix metalloproteinase 9 (MMP9) and integrin β3 in human endometria. Total RNA was extracted from endometrium biopsies of infertile women with tubal factor and PCOS (n = 6 each) and then analyzed by quantitative reverse-transcription polymerase chain reaction. The values are presented as mean ± SEM. **P < .01 and *P < .05 (unpaired t-test). (b,c) IGFBP-1 and LIF protein expression. Tissue lysates (80 mg) were extracted from endometrial tissues of infertile women with tubal factor (control; n = 6) and women with PCOS (PCOS; n = 6), and then subjected to Western blot analysis with the use of anti-LIF, anti-IGFBP-1 or anti-β-actin antibody, as described in the Materials and Methods. Left figure shows one representative experiment among three separate experiments. Right histogram shows relative protein levels of IGFBP-1 and LIF normalized to β-actin (Right). The values are presented as mean ± SEM. *P < .05 (unpaired t-test).

Article Snippet: In brief, the separated samples were transferred to polyvinylidene difluoride membranes and incubated overnight at 4 °C with rabbit polyclonal anti-α-IGFBP-1 (1:1000;13981-1-AP, Proteintech), anti-LIF (1:1000; 26757-1-AP, Proteintech), and human polyclonal anti-βactin (1:0000; 66031-1-Ig Proteintech).

Techniques: Expressing, Binding Assay, Reverse Transcription, Polymerase Chain Reaction, Control, Western Blot